Anticorps Polyclonal de lapin anti-MMP20

MMP20 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 55467-1-AP

Synonymes

Enamel metalloproteinase, Enamelysin, matrix metallopeptidase 20, Matrix metalloproteinase 20, MMP 20, MMP20

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Galerie de données de validation

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  WB analysis of mouse brain using 55467-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55467-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 55467-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 55467-1-AP (MMP20 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 55467-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55467-1-AP (MMP20 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 55467-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 55467-1-AP (MMP20 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 55467-1-AP

  Immunofluorescent analysis of HeLa cells using 55467-1-AP (MMP20 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

55467-1-AP cible MMP20 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: matrix metallopeptidase 20
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: NM_004771
• Symbole du gène: MMP20
• Identification du gène (NCBI): 9313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP20 belongs to the peptidase M10A family. It degrades amelogenin, the major protein component of the enamel matrix and two of the macromolecules characterizing the cartilage extracellular matrix: aggrecan and the cartilage oligomeric matrix protein (COMP). MMP20 may play a central role in tooth enamel formation. Defects in MMP20 are the cause of amelogenesis imperfecta hypomaturation type 2A2 (AI2A2). This antibody is specific to MMP20.

Publications

Species	Application	Title
human	IF	Int J Mol Sci; Overexpression of miR-1306-5p, miR-3195, and miR-3914 Inhibits Ameloblast Differentiation through Suppression of Genes Associated with Human Amelogenesis Imperfecta.; Authors - Hiroki Yoshioka
mouse	WB	Front Genet; Crucial Roles of microRNA-16-5p and microRNA-27b-3p in Ameloblast Differentiation Through Regulation of Genes Associated With Amelogenesis Imperfecta.; Authors - Akiko Suzuki
rat	WB	Cell Prolif; Smurf1 regulates ameloblast polarization by ubiquitination-mediated degradation of RhoA; Authors - Haoman Niu