Anticorps Polyclonal de lapin anti-MMP25

MMP25 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30655-1-PBS

Synonymes

MTMMP6, MT6MMP, MT MMP6, MMP 25, matrix metallopeptidase 25

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Galerie de données de validation

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  WB analysis using 30655-1-AP (same clone as 30655-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30655-1-AP (MMP25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 30655-1-AP (same clone as 30655-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30655-1-AP (MMP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 30655-1-AP (same clone as 30655-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using MMP25 antibody (30655-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30655-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30655-1-PBS cible MMP25 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP25 Protéine recombinante Ag29304
• Nom complet: matrix metallopeptidase 25
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 38-40 kDa
• Numéro d’acquisition GenBank: NM_022468
• Symbole du gène: MMP25
• Identification du gène (NCBI): 64386
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MMP25, also known as MT6-MMP, is one of the two glycosylphosphatidylinositol anchored matrix metalloproteinases (MMPs) that have been suggested to play a role in pericellular proteolysis. MMP25 was originally cloned from human leukocytes and its mRNA is predominantly expressed in leukocytes (polymorphonuclear cells and monocytes) of normal tissues and in brain, colon, urothelial and prostate cancers (PMID: 17513868). MMP-25 can exaggerate inflammatory responses by inactivating the main antiproteinase, a1-proteinase inhibitor or serpin (PMID: 16584348). The band at 57-kDa we detected is likely to be active MT6-MMP and minor MT6-MMP degradation product of 38-kDa.

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