Anticorps Polyclonal de lapin anti-MMP3

• Phare
• Validé par KD/KO

MMP3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

181

Avis (1)

N° de cat : 17873-1-AP

Synonymes

CHDS6, Matrix metalloproteinase 3, MMP 3, MMP3, SL 1, STMY, STMY1, STR1, Stromelysin 1, Transin 1

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Galerie de données de validation

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  WB analysis using 17873-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17873-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 17873-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 17873-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 17873-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17873-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17873-1-AP (MMP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 17873-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 17873-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach cancer using 17873-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 17873-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 17873-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 17873-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17873-1-AP (MMP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 17873-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (17873-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A549 using 17873-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (17873-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

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Applications testées

• Résultats positifs en WB: cellules A2780, cellules A549, cellules HeLa, cellules MG U-87, cellules PC-12, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 151 publications below
• IHC: See 40 publications below
• IF: See 10 publications below
• FC: See 1 publications below

Informations sur le produit

17873-1-AP cible MMP3 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, Lapin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP3 Protéine recombinante Ag12359
• Nom complet: matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• Masse moléculaire calculée: 477 aa, 54 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: BC074869
• Symbole du gène: MMP3
• Identification du gène (NCBI): 4314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

Publications

Species	Application	Title
mouse,rat	WB,IF	Autophagy; Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration; Authors - Di Wang
human	IHC	Bone Res; FABP4 secreted by M1-polarized macrophages promotes synovitis and angiogenesis to exacerbate rheumatoid arthritis.; Authors - Dong Guo
human	WB	Mater Today Bio; Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction; Authors - Zhanqiu Dai
rat	WB	Phytomedicine; Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells; Authors - Dong Wang
human	WB	Clin Cancer Res; BATF2 Deficiency Promotes Progression in Human Colorectal Cancer via Activation of HGF/MET Signaling: A Potential Rationale for Combining MET Inhibitors with IFNs.; Authors - Zebing Liu
mouse		Gut Microbes; Gut microbiota regulate tumor metastasis via circRNA/miRNA networks.; Authors - Zhuxian Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ana Luísa (Verified Customer) (07-25-2022)

Permeabilization: 0,3% Triton X-100 Blocking 2,5% BSA + 0,1% Triton X-100

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Intervertebral disc cells

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