Anticorps Monoclonal anti-MMP3

• Phare
• Validé par KD/KO

MMP3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F5A9

N° de cat : 66338-1-PBS

Synonymes

1F5A9, EC:3.4.24.17, Matrix metalloproteinase-3, MMP 3, MMP-3

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Galerie de données de validation

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  WB analysis of HeLa using 66338-1-Ig (same clone as 66338-1-PBS)

  WB result of MMP3 antibody (66338-1-Ig; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-MMP3 transfected HeLa cells. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  WB analysis using 66338-1-Ig (same clone as 66338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66338-1-Ig (same clone as 66338-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66338-1-Ig (same clone as 66338-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66338-1-Ig (same clone as 66338-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  WB analysis of pig heart using 66338-1-Ig (same clone as 66338-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  WB analysis of Caco-2 using 66338-1-Ig (same clone as 66338-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66338-1-Ig (same clone as 66338-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66338-1-Ig (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  IHC staining of rat colon using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66338-1-Ig (MMP3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66338-1-Ig (same clone as 66338-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using MMP3 antibody (66338-1-Ig, Clone: 1F5A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66338-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66338-1-PBS cible MMP3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MMP3 Protéine recombinante Ag12359
• Nom complet: matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• Masse moléculaire calculée: 477 aa, 54 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: BC074869
• Symbole du gène: MMP3
• Identification du gène (NCBI): 4314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Matrix metalloproteinases (MMPs) play a critically important role in extracellular matrix remodeling and have been implicated in a number of key normal and pathologic processes.These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 activate procollagenase via two pathways: slow direct activation and rapid activation in conjunction with tissue or plasma proteinases. The pro-MMP3 (60 kDa) and the active MMP3 (47 kDa) can be detected through western blot.

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