Anticorps Recombinant de lapin anti-MMP3

MMP3 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230535G8

N° de cat : 82984-4-PBS

Synonymes

230535G8, EC:3.4.24.17, Matrix metalloproteinase-3, MMP 3, MMP-3

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Galerie de données de validation

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  WB analysis using 82984-4-RR (same clone as 82984-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82984-4-RR (MMP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82984-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82984-4-RR (same clone as 82984-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug MMP3 Recombinant antibody (82984-4-RR, Clone:230535G8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82984-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82984-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82984-4-RR against Human MMP3 were performed. The affinity constant is 0.348 nM.

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Informations sur le produit

82984-4-PBS cible MMP3 dans les applications de WB, FC (Intra), Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MMP3 Protéine recombinante Eg0382
• Nom complet: matrix metallopeptidase 3 (stromelysin 1, progelatinase)
• Numéro d’acquisition GenBank: BC074815
• Symbole du gène: MMP3
• Identification du gène (NCBI): 4314
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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