Anticorps Polyclonal de lapin anti-MMP8

• Phare
• Validé par KD/KO

MMP8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 17874-1-AP

Synonymes

CLG1, EC:3.4.24.34, HNC, Matrix metalloproteinase 8, Matrix metalloproteinase-8

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Galerie de données de validation

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  WB analysis of HepG2 using 17874-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17874-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17874-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17874-1-AP (MMP8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17874-1-AP (MMP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17874-1-AP (MMP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17874-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17874-1-AP (MMP8 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17874-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MMP8 antibody (17874-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules Jurkat, tissu hépatique de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu ovarien humain, tissu rénal de souris, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

17874-1-AP cible MMP8 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MMP8 Protéine recombinante Ag12367
• Nom complet: matrix metallopeptidase 8 (neutrophil collagenase)
• Masse moléculaire calculée: 467 aa, 53 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC074988
• Symbole du gène: MMP8
• Identification du gène (NCBI): 4317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMP8(Matrix metalloproteinase-8) an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells(PMID:15367487). Purified human neutrophil MMP-8 with proeMMP-8 at 70-75 KDa and active enzyme at 65 KDa and amniochorion proenzyme and active enzyme at 65 and 50 KDa, respectively(PMID: 28772283, PMID:15042023).

Publications

Species	Application	Title
mouse	WB	PLoS Pathog; Circumventing Y. pestis Virulence by Early Recruitment of Neutrophils to the Lungs during Pneumonic Plague.; Authors - Yaron Vagima
mouse,human	WB,IHC	Stem Cell Res Ther; Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model.; Authors - Yihui Fan
human	WB,IHC	Am J Pathol; Preeclampsia is associated with alterations in DNA methylation of genes involved in collagen metabolism.; Authors - Mousa Ahmad A AA
mouse	IF	Viruses; Matrix Metalloproteinases Expression Is Associated with SARS-CoV-2-Induced Lung Pathology and Extracellular-Matrix Remodeling in K18-hACE2 Mice.; Authors - Hila Gutman
human	WB	Mediators Inflamm; Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes.; Authors - Congshan Jiang
rat	WB	Exp Eye Res; Luteolin ameliorates cornea stromal collagen degradation and inflammatory damage in rats with corneal alkali burn; Authors - Heng Wang

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