Anticorps Recombinant de lapin anti-MMP-9

MMP-9 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

241224B5

N° de cat : 82685-16-PBS

Synonymes

MMP9, GELB, EC:3.4.24.35, CLG4B, 82 kDa matrix metalloproteinase-9

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP01003-2

  Sandwich ELISA standard curve of MP01003-2, Human MMP-9 Recombinant Matched Antibody Pair - PBS only. 82685-18-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0442. 82685-16-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

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  Sample test of MP01003-2

  Serum of sixteen individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 213.0 ng/mL with a range of 67.5 - 426.4 ng/mL.

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  Sample test of MP01003-2

  Saliva of eight individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 80.7 ng/mL with a range of 1.9 - 260.9 ng/mL.

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  Sample test of MP01003-2

  Urine of eight individual healthy human donors was measured. The MMP-9 concentration of detected samples was determined to be 207.3 pg/mL with a range of 11.4 - 841.8 pg/mL.

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  Sample test of MP01003-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. The mean MMP-9 concentration was determined to be 157.3 ng/mL in unstimulated PBMC supernatant, 237.9 ng/mL in PHA stimulated PBMC supernatant.

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  Cytometric bead array standard curve of MP01003-2

  Cytometric bead array standard curve of MP01003-2, MMP-9 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82685-18-PBS. Detection antibody: 82685-16-PBS. Standard: Eg0442. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 82685-16-PBS

  Biolayer interferometry (BLl) kinetic assay of 82685-16-PBS against Human MMP-9 was performed. The affinity constant is 0.726 nM.

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Informations sur le produit

82685-16-PBS cible MMP-9 dans les applications de Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• Masse moléculaire calculée: 707 aa, 78 kDa
• Numéro d’acquisition GenBank: BC006093
• Symbole du gène: MMP-9
• Identification du gène (NCBI): 4318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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