Anticorps Monoclonal anti-MMS19

• Phare
• Validé par KD/KO

MMS19 Monoclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

2H6D8

N° de cat : 66049-1-Ig

Synonymes

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

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Galerie de données de validation

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  WB analysis of HeLa using 66049-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66049-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66049-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66049-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 66049-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66049-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 66049-1-Ig

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66049-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66049-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66049-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66049-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 66049-1-Ig

  IP Result of anti-MMS19 (IP:66049-1-Ig, 4ug; Detection:66049-1-Ig 1:2000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 66049-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66049-1-Ig(MMS19 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 66049-1-Ig

  Immunofluorescent analysis of fixed Hela cells using 66049-1-Ig (MMS19 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules NIH/3T3, cellules RAW 264.7, tissu cérébral de rat, tissu cérébral humain, tissu cérébral humain fœtal, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66049-1-Ig cible MMS19 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MMS19 Protéine recombinante Ag8960
• Nom complet: MMS19 nucleotide excision repair homolog (S. cerevisiae)
• Masse moléculaire calculée: 1030 aa, 113 kDa
• Poids moléculaire observé: 103 kDa, 38 kDa
• Numéro d’acquisition GenBank: BC006575
• Symbole du gène: MMS19
• Identification du gène (NCBI): 64210
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.

Publications

Species	Application	Title
human	WB	Science; MMS19 assembles iron-sulfur proteins required for DNA metabolism and genomic integrity.; Authors - Oliver Stehling ; KD Validated
human	WB	Mol Cell; An Oxygen-Dependent Interaction between FBXL5 and the CIA-Targeting Complex Regulates Iron Homeostasis.; Authors - Adarsh K Mayank
human	WB	Cancer Biomark; MMS19 as a potential predictive marker of adjuvant chemotherapy benefit in resected non-small cell lung cancer.; Authors - Julien Adam ; KD Validated
human	WB,IHC,IF	Aging (Albany NY); Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression; Authors - Guoyang Zhang