Anticorps Monoclonal anti-MMS19

• Phare
• Validé par KD/KO

MMS19 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H6D8

N° de cat : 66049-1-PBS

Synonymes

hMMS19, MET18, MET18 homolog, MMS19, MMS19 like protein, MMS19L

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Galerie de données de validation

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  WB analysis of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66049-1-Ig (same clone as 66049-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66049-1-Ig (same clone as 66049-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66049-1-Ig (same clone as 66049-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66049-1-Ig (same clone as 66049-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66049-1-Ig (same clone as 66049-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66049-1-Ig (same clone as 66049-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66049-1-Ig (same clone as 66049-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 66049-1-Ig (same clone as 66049-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66049-1-Ig (same clone as 66049-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66049-1-Ig (same clone as 66049-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66049-1-Ig (MMS19 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 66049-1-Ig (same clone as 66049-1-PBS)

  IP result of anti-MMS19 (IP:66049-1-Ig, 4ug; Detection:66049-1-Ig 1:2000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66049-1-Ig (MMS19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 66049-1-Ig (same clone as 66049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66049-1-Ig (MMS19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66049-1-Ig (same clone as 66049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66049-1-Ig(MMS19 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66049-1-Ig (same clone as 66049-1-PBS)

  Immunofluorescent analysis of fixed Hela cells using 66049-1-Ig (MMS19 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66049-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66049-1-PBS cible MMS19 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MMS19 Protéine recombinante Ag8960
• Nom complet: MMS19 nucleotide excision repair homolog (S. cerevisiae)
• Masse moléculaire calculée: 1030 aa, 113 kDa
• Poids moléculaire observé: 103 kDa, 38 kDa
• Numéro d’acquisition GenBank: BC006575
• Symbole du gène: MMS19
• Identification du gène (NCBI): 64210
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MMS19 (MMS19 nucleotide excision repair homolog), also known as MET18, is a 1,030 amino acid nuclear protein containing seven HEAT repeats that belongs to the MET18/MMS19 family. Via its interactions with TFIIH p80 and TFIIH p89 helicases, MMS19 plays a role in nucleotide excision repair (NER) and RNA polymerase II (Pol II) transcription. MMS19 may also function as a transcriptional coactivator of estrogen receptor. While ubiquitously expressed, highest levels of MMS19 have been found in testis. At least five distinct MMS19 protein isoforms exist, which are produced by alternative splicing events.

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