Anticorps Polyclonal de lapin anti-MND1

• Phare
• Validé par KD/KO

MND1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 11636-1-AP

Synonymes

GAJ, MND1

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Galerie de données de validation

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  WB analysis of human liver using 11636-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 11636-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11636-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11636-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11636-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11636-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11636-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11636-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11636-1-AP (MND1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11636-1-AP

  IP result of anti-MND1 (IP:11636-1-AP, 4ug; Detection:11636-1-AP 1:600) with mouse liver tissue lysate 6000ug.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules MCF-7, tissu hépatique de souris, tissu hépatique humain, tissu pulmonaire humain, tissu rénal humain
• Résultats positifs en IP: tissu hépatique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11636-1-AP cible MND1 dans les applications de WB, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MND1 Protéine recombinante Ag2210
• Nom complet: meiotic nuclear divisions 1 homolog (S. cerevisiae)
• Masse moléculaire calculée: 205 aa, 24 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC032142
• Symbole du gène: MND1
• Identification du gène (NCBI): 84057
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IF	Oncotarget; A study of meiomitosis and novel pathways of genomic instability in cutaneous T-cell lymphomas (CTCL).; Authors - Matthew Tsang
human	WB	Med Oncol; Meiotic nuclear divisions 1 promotes proliferation and metastasis in hepatocellular carcinoma and is a potential diagnostic and therapeutic target gene; Authors - Kai Tan ; KD Validated
human	WB	J Inflamm Res; Integrative Pan-Cancer Analysis Reveals the Oncogenic Role of MND1 and Validation of MND1's Role in Breast Cancer; Authors - Wenwu Zhang ; KD Validated

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