Anticorps Recombinant de lapin anti-MNT

• Phare
• Validé par KD/KO

MNT Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240550A10

N° de cat : 83648-1-RR

Synonymes

240550A10, bHLHd3, Class D basic helix-loop-helix protein 3, MAD6, MAX binding protein

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Galerie de données de validation

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  WB analysis using 83648-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83648-1-RR (MNT antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 83648-1-RR

  WB result of MNT antibody (83648-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MNT transfected HeLa cells.

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  IF Staining of HeLa using 83648-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MNT antibody (83648-1-RR, Clone: 240550A10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of A431 using 83648-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MNT Recombinant antibody (83648-1-RR, Clone:240550A10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 83648-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83648-1-RR against Human MNT were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A431, cellules C6, cellules HEK-293T, cellules HeLa, cellules HT-29, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83648-1-RR cible MNT dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MNT Protéine recombinante Ag34529
• Nom complet: MAX binding protein
• Masse moléculaire calculée: 582 aa, 62 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC117563
• Symbole du gène: MNT
• Identification du gène (NCBI): 4335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mnt (Max binding protein), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein that forms a complex with Max (Myc-associated factor X) to repress transcription. Mnt contains one basic helix-loop-helix (bHLH) domain and is encoded by a gene that maps to human chromosome 17p13.3. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Tumor suppressor p53 is necessary for maintenance of cellular genetic integrity by moderating cell fate through DNA repair versus cell death. Like p53, BRCA1 is directly involved in DNA repair, though specifically it is recognized as a genetic determinant of early onset breast cancer and predisposition to cancers of the ovary, colon, prostate gland and fallopian tubes.

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