Anticorps Polyclonal de lapin anti-MOG

MOG Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

19

Avis (1)

N° de cat : 12690-1-AP

Synonymes

MOG, MOGIG 2, myelin oligodendrocyte glycoprotein

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Galerie de données de validation

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  WB analysis using 12690-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12690-1-AP (MOG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12690-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12690-1-AP (MOG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12690-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12690-1-AP (MOG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

12690-1-AP cible MOG dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MOG Protéine recombinante Ag3273
• Nom complet: myelin oligodendrocyte glycoprotein
• Masse moléculaire calculée: 295 aa, 34 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC035938
• Symbole du gène: MOG
• Identification du gène (NCBI): 4340
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myelin/oligodendrocyte glycoprotein (MOG), a 23~28 kDa glycoprotein, a myelin antigen at the outer surface of the central nervous system (CNS) myelin sheath, which may trigger T-cell as well as B-cell responses. It therefore constitutes a pivotal target for autoimmune responses, which result in inflammation and also demyelination in the CNS. Its presence on the outer- most lamellae of mature CNS myelin and its late appearance during myelinogenesis suggest that it contributes to myelin maturation or maintenance. 10 isoforms of MOG produced by alternative splicing have been described, and heterodimers may be formed between the different isoforms. Defects in MOG are the cause of narcolepsy type 7 (NRCLP7), a neurological disabling sleep disorder characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, cataplexy, hypnagogic hallucinations, and sleep paralysis. Role of MOG in the pathogenesis of multiple sclerosis (MS) has been reported but remains to be clarified.

Publications

Species	Application	Title
mouse	IF	Elife; Activity-dependent modulation of synapse-regulating genes in astrocytes.; Authors - Isabella Farhy-Tselnicker
human	IHC	Int J Mol Sci; The Distribution of GPR17-Expressing Cells Correlates with White Matter Inflammation Status in Brain Tissues of Multiple Sclerosis Patients.; Authors - Jacopo Angelini
mouse	WB	Glia; ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.; Authors - Lukasz Mateusz Szewczyk
human	IHC	J Neuroinflammation; Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain.; Authors - Gloria Donninelli
human	IHC	Brain Pathol; Overexpression of the ubiquitin-editing enzyme A20 in the brain lesions of Multiple Sclerosis patients: moving from systemic to central nervous system inflammation.; Authors - Simona Perga
human		J Virol; Identification of the myelin oligodendrocyte glycoprotein as a cellular receptor for rubella virus.; Authors - Cong Haolong H

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

MOG (in red) worked nicely showing the mielinated axons in my paraffin human brain cortex sections in IF

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

MOG Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:12690-1-AP)

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