Anticorps Polyclonal de lapin anti-GCS1

• Phare
• Validé par KD/KO

GCS1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 17859-1-AP

Synonymes

GCS1, glucosidase I, MOGS, Processing A glucosidase I

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Galerie de données de validation

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  WB analysis using 17859-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 17859-1-AP (GCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17859-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17859-1-AP (GCS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 17859-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human small intestine using 17859-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 17859-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17859-1-AP (GCS1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 17859-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17859-1-AP (GCS1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human small intestine using 17859-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 17859-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine using 17859-1-AP (GCS1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules COLO 320,
• Résultats positifs en IHC: tissu d'intestin grêle humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

17859-1-AP cible GCS1 dans les applications de WB, IF, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GCS1 Protéine recombinante Ag12321
• Nom complet: mannosyl-oligosaccharide glucosidase
• Masse moléculaire calculée: 837 aa, 92 kDa
• Poids moléculaire observé: 80-92 kDa
• Numéro d’acquisition GenBank: BC028337
• Symbole du gène: GCS1
• Identification du gène (NCBI): 7841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MOGS (Mannosyl-oligosaccharide glucosidase), also known as GCS1, is the first enzyme in the N-linked oligosaccharide processing pathway. The enzyme cleaves the distal alpha-1,2-linked glucose residue from the Glc(3)-Man(9)-GlcNAc(2) oligosaccharide precursor (PMID: 38187061). The resulting Glc(2)-Man(9)-GlcNAc(2) intermediate is then further modified by glucosidase II and several ER- and Golgi-resident mannosidases and glycosyltransferases, finally yielding a complex array of glycan structures (PMID: 3896128). MOGS has two isoforms, and it is located in the lumen of the endoplasmic reticulum.

Publications

Species	Application	Title
rat	WB	J Cell Biol; Competition for calnexin binding regulates secretion and turnover of misfolded GPI-anchored proteins; Authors - Amber M Cheatham
human	WB	PLoS Pathog; Identification of cell type specific ACE2 modifiers by CRISPR screening.; Authors - Emily J Sherman
	WB	Int J Mol Sci; The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts.; Authors - Jianyu Ma
human	WB,IHC	Front Genet; Subtypes analysis and prognostic model construction based on lysosome-related genes in colon adenocarcinoma; Authors - Yang Chen
human	WB	Am J Physiol Cell Physiol; Hydrogen Sulfide Accumulates LDL Receptor Precursor via Downregulating PCSK9 in HepG2 Cells.; Authors - Yong Huang
human	IF	Neurogenetics; Identification and characterization of novel mutations in MOGS in a Chinese patient with infantile spams.; Authors - Xuehua Peiwei Zhao

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