Anticorps Monoclonal anti-MON1B

• Phare
• Validé par KD/KO

MON1B Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3E7C6

N° de cat : 67985-1-PBS

Synonymes

HSRG1, KIAA0872, MON1 homolog B (yeast), MON1B, SAND2

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Galerie de données de validation

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  WB analysis using 67985-1-Ig (same clone as 67985-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67985-1-Ig (MON1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67985-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 67985-1-Ig (same clone as 67985-1-PBS)

  WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected A549 cells. This data was developed using the same antibody clone with 67985-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67985-1-Ig (same clone as 67985-1-PBS)

  WB result of MON1B antibody (67985-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MON1B transfected HeLa cells. This data was developed using the same antibody clone with 67985-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67985-1-Ig (same clone as 67985-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MON1B antibody (67985-1-Ig, Clone: 3E7C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67985-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67985-1-PBS cible MON1B dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MON1B Protéine recombinante Ag9920
• Nom complet: MON1 homolog B (yeast)
• Masse moléculaire calculée: 547 aa, 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC024277
• Symbole du gène: MON1B
• Identification du gène (NCBI): 22879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Vacuolar fusion protein MON1 homolog B (MON1B) is also named as HSRG1, KIAA0872 and SAND2, and belongs to the MON1/SAND family. The membrane recruitment of MON1B, a homolog of yeast MON1 protein in mammals, is essential for vesicular fusion of early endosomes (PMID:26987402).

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