Anticorps Polyclonal de lapin anti-MORC3

MORC3 Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-24994

Synonymes

ZCWCC3, ZCW5, NXP2, Nuclear matrix protein 2, MORC family CW-type zinc finger protein 3

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Galerie de données de validation

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  IF Staining of A431 using CL488-24994

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 MORC3 antibody (CL488-24994) at dilution of 1:200, CL594-phalloidin (red).

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  FC experiment of A431 using CL488-24994

  1x10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MORC3 Polyclonal antibody (CL488-24994)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en IF/ICC: cellules A431,
• Résultats positifs en FC (Intra): cellules A431,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-24994 cible MORC3 dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MORC3 Protéine recombinante Ag21825
• Nom complet: MORC family CW-type zinc finger 3
• Masse moléculaire calculée: 939 aa, 107 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC132731
• Symbole du gène: MORC3
• Identification du gène (NCBI): 23515
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MORC3, also named as MORC family CW-type zinc finger protein 3 or NXP2, is a 939 amino acid protein, which contains 1 CW-type zinc finger. MORC3 localizes in the nucleus and are expressed in heart, placenta, skeletal muscle, brain, pancreas, lung, liver, but not kidney. MORC3 as Nuclear factor which forms MORC3-NBs (nuclear bodies) via an ATP-dependent mechanism. Sumoylated MORC3-NBs can also associate with PML-NBs and recruits TP53 and SP100 to PML-NBs, thus regulating TP53 activity. It may be required for influenza A transcription during viral infection. The calculated molecular weight of MORC3 is a 107 kDa, but the Sumoylated MORC3 protein is about 130 kDa.

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