Anticorps Polyclonal de lapin anti-MORF4L1

MORF4L1 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 55257-1-AP

Synonymes

Eaf3, FWP006, HSPC008, HSPC061, HsT17725, MORF related gene 15 protein, MORF4L1, MORFRG15, mortality factor 4 like 1, MRG15, PP368, Protein MSL3 1, S863 6

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Galerie de données de validation

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  WB analysis of HEK-293 using 55257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55257-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55257-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 55257-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55257-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55257-1-AP (MORF4L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 55257-1-AP

  IP result of anti-MORF4L1 (IP:55257-1-AP, 4ug; Detection:55257-1-AP 1:800) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 55257-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55257-1-AP (MORF4L1 antibody) at dilution of 1:200 (under 10x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, tissu cérébral de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cutané humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

55257-1-AP cible MORF4L1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mortality factor 4 like 1
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: NM_006791
• Symbole du gène: MORF4L1
• Identification du gène (NCBI): 10933
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MORF4L1, also named as MRG15, belongs to the MRG family. It is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. It is also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. (PMID:14966270) This antibody has no cross-reaction to MORF4L2.

Publications

Species	Application	Title
human	WB	bioRxiv; Dynamic Regulation OF The Chromatin Environment By Ash1L Modulates Human Neuronal Structure And Function; Authors - Megha Jhanji

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