Anticorps Polyclonal de lapin anti-MOV10

• Phare
• Validé par KD/KO

MOV10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(28)

N° de cat : 10370-1-AP

Synonymes

Armitage homolog, EC:3.6.4.13, fSAP113, gb110, Helicase MOV-10

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Galerie de données de validation

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  WB analysis using 10370-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10370-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 10370-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10370-1-AP (MOV10 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 10370-1-AP

  IP result of anti-MOV10 (IP:10370-1-AP, 4ug; Detection:10370-1-AP 1:5000) with HepG2 cells lysate 1480 ug.

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  IHC staining of human prostate hyperplasia using 10370-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10370-1-AP (MOV10 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 10370-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10370-1-AP (MOV10 antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 10370-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MOV10 antibody (10370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 10370-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MOV10 antibody (10370-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 8 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

10370-1-AP cible MOV10 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MOV10 Protéine recombinante Ag0384
• Nom complet: Mov10, Moloney leukemia virus 10, homolog (mouse)
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 110-115 kDa
• Numéro d’acquisition GenBank: BC002548
• Symbole du gène: MOV10
• Identification du gène (NCBI): 4343
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals(PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages(PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

Publications

Species	Application	Title
human	WB	Neuron; A coordinated local translational control point at the synapse involving relief from silencing and MOV10 degradation.; Authors - Banerjee Sourav S
		Mol Cell; Multivalent Proteins Rapidly and Reversibly Phase-Separate upon Osmotic Cell Volume Change.; Authors - Ameya P Jalihal
human	WB	Mol Cell; MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs.; Authors - Lea H Gregersen ; KD Validated
human	WB,IP	Nat Struct Mol Biol; Capped small RNAs and MOV10 in human hepatitis delta virus replication.; Authors - Haussecker Dirk D ; KD Validated
human	WB,IF	PLoS Genet; MOV10 RNA helicase is a potent inhibitor of retrotransposition in cells.; Authors - Goodier John L JL
human	IF	PLoS Pathog; APOBEC3G and APOBEC3F require an endogenous cofactor to block HIV-1 replication.; Authors - Han Yanxing Y

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