Anticorps Recombinant de lapin anti-MOV10

MOV10 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250130D2

N° de cat : 85818-5-RR

Synonymes

Armitage homolog, EC:3.6.4.13, fSAP113, gb110, Helicase MOV-10

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Galerie de données de validation

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  WB analysis using 85818-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85818-5-RR (MOV10 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 85818-5-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MOV10 antibody (85818-5-RR, Clone: 250130D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85818-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85818-5-RR against Human MOV10 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules C6, cellules HEK-293, cellules HeLa, cellules L02, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85818-5-RR cible MOV10 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MOV10 Protéine recombinante Ag0384
• Nom complet: Mov10, Moloney leukemia virus 10, homolog (mouse)
• Masse moléculaire calculée: 114 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC002548
• Symbole du gène: MOV10
• Identification du gène (NCBI): 4343
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MOV10, also named as KIAA1631, belongs to the DNA2/NAM7 helicase family and SDE3 subfamily. It is required for RNA-mediated gene silencing by the RNA-induced silencing complex (RISC). Human MOV10 may regulate a wide range of RNA viruses and could also control the retrotransposition of endogenous retroelements in mammals (PMID:22727223). MOV10 has a broad antiretroviral activity that can target a wide range of retroviruses, and it could be actively involved in host defense against retroviral infection. MOV10 can potently inhibit HIV-1 replication at multiple stages (PMID:20215113). It is involved in the progression of telomerase-catalyzing reaction via the interaction of telomerase protein and telomere DNA.

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