Anticorps Polyclonal de lapin anti-MPG

MPG Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11481-2-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 11481-2-AP (same clone as 11481-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 11481-2-AP (same clone as 11481-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 11481-2-AP (same clone as 11481-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11481-2-AP (MPG antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 11481-2-AP (same clone as 11481-2-PBS)

  IP result of anti-MPG (IP:11481-2-AP, 4ug; Detection:11481-2-AP 1:300) with HepG2 cells lysate 2200 ug. This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11481-2-AP (same clone as 11481-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11481-2-AP (MPG antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11481-2-PBS in a different storage buffer formulation.

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Informations sur le produit

11481-2-PBS cible MPG dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPG Protéine recombinante Ag2034
• Nom complet: N-methylpurine-DNA glycosylase
• Masse moléculaire calculée: 293 aa, 32 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC014991
• Symbole du gène: MPG
• Identification du gène (NCBI): 4350
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

N-methylpurine DNA glycosylase (MPG), an initiator glycosylase of the base excision repair (BER) pathway, typically repairs alkyl-induced DNA adducts, many of which are associated with genomic instability and tumorigenic risk [PMID:22801474] MPG initiates BER by removing TMZ-induced N3-methyladenine and N7-methylguanine base lesions, leaving abasic sites (AP sites) in DNA for further processing by BER [PMID:21377995]. MPG involves in modulating the genotoxic threshold response induced by the Sn2 alkylating agent ethyl methanesulfonate (EMS) [PMID:21071726].

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