Anticorps Polyclonal de lapin anti-MPI

• Phare
• Validé par KD/KO

MPI Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 14234-1-AP

Synonymes

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

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Galerie de données de validation

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  WB analysis using 14234-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14234-1-AP (MPI antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14234-1-AP

  WB result of MPI antibody (14234-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPI transfected HEK-293 cells.

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  WB analysis of human brain using 14234-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14234-1-AP (MPI antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14234-1-AP

  IP result of anti-MPI (IP:14234-1-AP, 4ug; Detection:14234-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IF Staining of NIH/3T3 using 14234-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 14234-1-AP (MPI antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of NIH/3T3 using 14234-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using MPI antibody (14234-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

14234-1-AP cible MPI dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPI Protéine recombinante Ag5475
• Nom complet: mannose phosphate isomerase
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 40-47 kDa
• Numéro d’acquisition GenBank: BC046357
• Symbole du gène: MPI
• Identification du gène (NCBI): 4351
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nature; Mannose impairs tumour growth and enhances chemotherapy.; Authors - Pablo Sierra Gonzalez ; KD Validated
rat	WB	Mil Med Res; D-mannose alleviates intervertebral disc degeneration through glutamine metabolism; Authors - Zheng-Lin Dong
human	WB	Int J Biol Sci; Mannose Inhibits NSCLC Growth and Inflammatory Microenvironment by Regulating Gut Microbiota and Targeting OGT/hnRNP R/JUN/IL-8 Axis; Authors - Haoyi Jin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Macarena Lucia (Verified Customer) (10-17-2022)

clear band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

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