Anticorps Recombinant de lapin anti-MPO

• Phare
• Validé par KD/KO

MPO Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

3J17

N° de cat : 81610-1-RR

Synonymes

3J17, EC:1.11.2.2, myeloperoxidase

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Galerie de données de validation

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  WB analysis of HL-60 using 81610-1-RR

  HL-60 cells were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human saliva using 81610-1-RR

  human saliva were subjected to SDS PAGE followed by western blot with 81610-1-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 81610-1-RR

  WB result of MPO antibody (81610-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPO transfected HL-60 cells.

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  IF Staining of HL-60 using 81610-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HL-60 cells using MPO antibody (81610-1-RR, Clone: 3J17 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HL-60 using 81610-1-RR

  1x10^6 HL-60 cells were intracellularly stained with 0.25 ug MPO Recombinant antibody (81610-1-RR, Clone:3J17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 81610-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81610-1-RR against Human MPO were performed. The affinity constant is 3.87 nM.

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Applications testées

• Résultats positifs en WB: cellules HL-60, salive humain, salive humaine
• Résultats positifs en IF/ICC: cellules HL-60,
• Résultats positifs en FC (Intra): cellules HL-60,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:800-1:3200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

81610-1-RR cible MPO dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MPO Protéine recombinante Ag17564
• Nom complet: myeloperoxidase
• Masse moléculaire calculée: 745 aa, 84 kDa
• Poids moléculaire observé: 59 kDa, 90 kDa
• Numéro d’acquisition GenBank: BC130476
• Symbole du gène: MPO
• Identification du gène (NCBI): 4353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244).The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IF	Placenta; NETs exacerbate placental inflammation and injury through high mobility group protein B1 during preeclampsia; Authors - Yurong Lu
mouse	IF	Free Radic Biol Med; Neutrophil extracellular traps induce fibroblast ferroptosis via IRE1α/XBP1-mediated ER stress to impair diabetic wound healing; Authors - Hongbo Zhao

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