Anticorps Recombinant de lapin anti-MPO

MPO Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240229A6

N° de cat : 83282-5-PBS

Synonymes

EC:1.11.2.2, myeloperoxidase

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00321-2

  Sandwich ELISA standard curve of MP00321-2, Human MPO Recombinant Matched Antibody Pair - PBS only. 83282-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0511. 83282-5-PBS was HRP conjugated as the detection antibody. Range: 39-2500 pg/mL.

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  Sample test of MP00321-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days or 5 days. For the PBMC supernatant cultured for 3 days, the mean MPO concentration was determined to be 6.6 ng/mL in unstimulated PBMC supernatant, 20.0 ng/mL in PHA stimulated PBMC supernatant. For the PBMC supernatant cultured for 5 days, the mean MPO concentration was determined to be 7.2 ng/mL in unstimulated PBMC supernatant, 28.2 ng/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP00321-2

  Urine of six individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 2,249.9 pg/mL with a range of 294.1 - 6,991.1 pg/mL.

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  Sample test of MP00321-2

  Saliva of eight individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 314.7 ng/mL with a range of 6.3 - 1,446.1 ng/mL.

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  Sample test of MP00321-2

  The mean MPO concentration was determined to be 73,583.3 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

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  Sample test of MP00321-2

  Serum of sixteen individual healthy human donors was measured. The MPO concentration of detected samples was determined to be 169.9 ng/mL with a range of 54.5 - 419.2 ng/mL.

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  Affinity and Kinetic Characterization of 83282-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 83282-5-PBS against Human MPO was performed. The affinity constant is 1.27 nM.

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Informations sur le produit

83282-5-PBS cible MPO dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MPO Protéine recombinante Eg0511
• Nom complet: myeloperoxidase
• Masse moléculaire calculée: 745 aa, 84 kDa
• Numéro d’acquisition GenBank: BC130476
• Symbole du gène: MPO
• Identification du gène (NCBI): 4353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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