Anticorps Recombinant de lapin anti-MPO

MPO Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241839C11

N° de cat : 84436-5-PBS

Synonymes

Myeloperoxidase light chain, Myeloperoxidase heavy chain, Myeloperoxidase, EC:1.11.2.2, 241839C11

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Galerie de données de validation

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  WB analysis using 84436-5-RR (same clone as 84436-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84436-5-RR (MPO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84436-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84436-5-RR (same clone as 84436-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84436-5-RR (MPO antibody) at dilution of 1:250 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84436-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 84436-5-RR (same clone as 84436-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84436-5-RR (MPO antibody) at dilution of 1:250 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84436-5-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 84436-5-RR (same clone as 84436-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using MPO antibody (84436-5-RR, Clone: 241839C11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84436-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84436-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84436-5-RR against Mouse Mpo were performed. The affinity constant is 23.5 nM.

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Informations sur le produit

84436-5-PBS cible MPO dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: myeloperoxidase
• Masse moléculaire calculée: 81KD
• Poids moléculaire observé: 43 kDa, 59 kDa, 90 kDa
• Numéro d’acquisition GenBank: AAR99349
• Symbole du gène: Mpo
• Identification du gène (NCBI): 17523
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The MPO gene encodes myeloperoxidase, a lysosomal hemoprotein located in the azurophilic granules of polymorphonuclear (PMN) leukocytes and monocytes. In response to stimulation, MPO is activated into a transient intermediate with potent antimicrobial oxidizing abilities(PMID:17650507). The mRNA is translated into a single protein of 90 kDa, which displays enzymatic activity and undergoes proteolytic maturation into a heavy chain of 59 kDa and a light chain of 13.5 kDa; these subunits then dimerize into the mature tetramer and the mature MPO is a heterotetramer composed of two identical heavy chains and two identical light chains(PMID:12773517). Fragments with molecular masses of 43-47 kDa were formed by autocatalysis during warming in sample buffer (PMID:12960244). The 24-kDa material had a map identical to that of 13.5 kDa subunit and represents a dimer of the 13.5 kDa subunit (PMID:3008892). Defects in MPO are the cause of myeloperoxidase deficiency (MPOD). It has 3 isoforms produced by alternative splicing.

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