Anticorps Polyclonal de lapin anti-MPP5

• Phare
• Validé par KD/KO

MPP5 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 17710-1-AP

Synonymes

MAGUK p55 subfamily member 5, MPP5, PALS1

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Galerie de données de validation

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  WB analysis using 17710-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17710-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17710-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17710-1-AP

  IP Result of anti-MPP5 (IP:17710-1-AP, 3ug; Detection:17710-1-AP 1:1000) with mouse brain tissue lysate 8000ug.

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  IHC staining of mouse brain using 17710-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17710-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules DU 145, cellules HeLa, cellules Y79, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 6 publications below
• IF: See 15 publications below
• IP: See 1 publications below

Informations sur le produit

17710-1-AP cible MPP5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPP5 Protéine recombinante Ag12010
• Nom complet: membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
• Masse moléculaire calculée: 675 aa, 77 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC095485
• Symbole du gène: MPP5
• Identification du gène (NCBI): 64398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.

Publications

Species	Application	Title
mouse	IF	Neuron; Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.; Authors - Divya Jayaraman
mouse	IF	Neuron; Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells.; Authors - Robert Beattie
human	WB,IP	Nat Commun; CD13 orients the apical-basal polarity axis necessary for lumen formation.; Authors - Li-Ting Wang
mouse	IF	Dev Cell; Multicellular rosettes link mesenchymal-epithelial transition to radial intercalation in the mouse axial mesoderm; Authors - Marissa L Gredler
mouse	IF	Cell Death Differ; The evolutionary history of the polyQ tract in huntingtin sheds light on its functional pro-neural activities.; Authors - Raffaele Iennaco
human	WB,IF	J Am Soc Nephrol; Crumbs2 Is an Essential Slit Diaphragm Protein of the Renal Filtration Barrier.; Authors - Annika Möller-Kerutt