Anticorps Polyclonal de lapin anti-MPP5

• Phare
• Validé par KD/KO

MPP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17710-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 17710-1-AP (same clone as 17710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  WB analysis using 17710-1-AP (same clone as 17710-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 17710-1-AP (same clone as 17710-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 17710-1-AP (MPP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

  IP result of anti-MPP5 (IP:17710-1-AP, 3ug; Detection:17710-1-AP 1:1000) with mouse brain tissue lysate 8000ug. This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17710-1-AP (same clone as 17710-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17710-1-AP (MPP5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 17710-1-AP (same clone as 17710-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MPP5 antibody (17710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17710-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17710-1-PBS cible MPP5 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPP5 Protéine recombinante Ag12010
• Nom complet: membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)
• Masse moléculaire calculée: 675 aa, 77 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC095485
• Symbole du gène: MPP5
• Identification du gène (NCBI): 64398
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MPP5, also named as PALS1, belongs to the MAGUK family which interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. MPP5 is thought to organize intracellular PALS1-CRB-MUPP1 protein scaffolds in the retina that are involved in maintenance of photoreceptor-Mu¨ller glia cell adhesion. MPP5 plays a role in tight junctions biogenesis and in the establishment of cell polarity in epithelial cells.

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