Anticorps Polyclonal de lapin anti-MPRIP

MPRIP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20040-1-AP

Synonymes

KIAA0864, M RIP, MPRIP, MRIP, p116Rip, Rho interacting protein 3, RHOIP3, RIP3

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 20040-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 20040-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 20040-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 20040-1-AP (MPRIP Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of SKOV-3 using 20040-1-AP

  IP Result of anti-MPRIP (IP:20040-1-AP, 4ug; Detection:20040-1-AP 1:500) with SKOV-3 cells lysate 2000ug.

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  IHC staining of human ovary using 20040-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 20040-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20040-1-AP (MPRIP antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse ovary using 20040-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 20040-1-AP (MPRIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 20040-1-AP

  Immunofluorescent analysis of Hela cells, using MPRIP antibody 20040-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules A2780, cellules SKOV-3
• Résultats positifs en IP: cellules SKOV-3
• Résultats positifs en IHC: tissu ovarien de souris, tissu ovarien humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

20040-1-AP cible MPRIP dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin phosphatase Rho interacting protein
• Masse moléculaire calculée: 117 kDa
• Poids moléculaire observé: 116-140 kDa
• Numéro d’acquisition GenBank: NM_015134
• Symbole du gène: MPRIP
• Identification du gène (NCBI): 23164
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MPRIP, also named as KIAA0864, MRIP, RHOIP3 and p116Rip, is required for the regulation of the actin cytoskeleton by RhoA and ROCK1. MRPIP targets myosin phosphatase to the actin cytoskeleton. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. The antibody recognizes the C-term of isoform2 and isoform3 of MPRIP.

Publications

Species	Application	Title
human	WB,IF	Glia; Ermin is a p116RIP -interacting protein promoting oligodendroglial differentiation and myelin maintenance.; Authors - Shan Wang
human	WB	RNA; DAP5 drives translation of specific mRNA targets with upstream ORFs in human embryonic stem cells; Authors - Maya David
human	WB	J Cancer; High Expression of lncRNA AFAP1-AS1 Promotes the Progression of Colon Cancer and Predicts Poor Prognosis.; Authors - Hao Bo