Anticorps Polyclonal de lapin anti-MPZ / P0

MPZ / P0 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10572-1-PBS

Synonymes

MPZ, Myelin protein zero, CHM, MPP, Myelin peripheral protein

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Galerie de données de validation

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  WB analysis using 10572-1-AP (same clone as 10572-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10572-1-AP (MPZ / P0 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

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  IHC staining of rat Ischiadic nerve using 10572-1-AP (same clone as 10572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat Ischiadic nerve tissue slide using 10572-1-AP (MPZ / P0 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

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  IHC staining of rat Ischiadic nerve using 10572-1-AP (same clone as 10572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat Ischiadic nerve tissue slide using 10572-1-AP (MPZ / P0 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse Ischiadic nerve using 10572-1-AP (same clone as 10572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse Ischiadic nerve tissue slide using 10572-1-AP (MPZ / P0 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse Ischiadic nerve using 10572-1-AP (same clone as 10572-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse Ischiadic nerve tissue slide using 10572-1-AP (MPZ / P0 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10572-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10572-1-PBS cible MPZ / P0 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MPZ / P0 Protéine recombinante Ag0848
• Nom complet: myelin protein zero
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC006491
• Symbole du gène: MPZ
• Identification du gène (NCBI): 4359
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MPZ (myelin protein zero), also known as P0, is a transmembrane glycoprotein that belongs to the immunoglobulin supergene family. Synthesized by myelin-forming Schwann cells, MPZ is the major structural protein component of myelin in the peripheral nervous system. It is involved in the formation and maintenance of compact myelin and plays a role in the creation of an extracellular membrane face which guides the wrapping process and ultimately compacts adjacent lamellae. More than 120 mutations detected in the gene of MPZ cause various forms of hereditary neuropathy, which include Charcot-Marie-Tooth disease type 1B (CMT1B), CMT2, Dejerine-Sottas syndrome (DSS), and congenital hypomyelination neuropathy (CHN). This antibody can recognize endogenous MPZ and can be used as a marker of myelinating Schwann cells. MPZ can be detected the 25-30 kDa band by western blot. A band of 36 kDa could also be detected, which is a novel isoform of MPZ that contains an additional domain at the C-terminal (PMID: 22457349). It can also exist as a dimer (PMID: 12933931).

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