Anticorps Polyclonal de lapin anti-MRE11

MRE11 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10744-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10744-1-AP (same clone as 10744-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10744-1-AP (MRE11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10744-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10744-1-AP (same clone as 10744-1-PBS)

  IP result of anti-MRE11 (IP:10744-1-AP, 4ug; Detection:10744-1-AP 1:600) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 10744-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 10744-1-AP (same clone as 10744-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10744-1-AP (MRE11 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 10744-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 10744-1-AP (same clone as 10744-1-PBS)

  Immunofluorescent analysis of A431 cells using 10744-1-AP (MRE11 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10744-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10744-1-PBS cible MRE11 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRE11 Protéine recombinante Ag1105
• Nom complet: MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: BC005241
• Symbole du gène: MRE11A
• Identification du gène (NCBI): 4361
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mre11 (meiotic recombination 11), also named as Mre11A, is a component of the MRN( MRE11/RAD50/NBS1) complex which is a versatile complex, playing a key role in the sensing, processing, and repair of DSBs. MRE11 possesses both endonuclease and 3'-5' exonuclease activity. It contains two DNA-binding domains (DBDs), enabling it to bind both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). After DSBs, MRE11 is loaded onto DSBs sites and cleaves DNA by cooperating with CtIP (CtBP [C terminal binding protein] interacting protein) to initiate end resection. It's reported that MRE11 is over-expressed in breast cancers. (PMID:38128537; 22914783)

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