Anticorps Polyclonal de lapin anti-MRP3/ABCC3

MRP3/ABCC3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25358-1-PBS

Synonymes

ABCC3, MRP3, ABC31, ATP-binding cassette sub-family C member 3, EC:7.6.2.-

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Galerie de données de validation

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  WB analysis using 25358-1-AP (same clone as 25358-1-PBS)

  37°C incubated HeLa cells were subjected to SDS PAGE followed by western blot with 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25358-1-AP (same clone as 25358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 25358-1-AP (same clone as 25358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 25358-1-AP (same clone as 25358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25358-1-AP (MRP3/ABCC3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25358-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25358-1-PBS cible MRP3/ABCC3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRP3/ABCC3 Protéine recombinante Ag19786
• Nom complet: ATP-binding cassette, sub-family C (CFTR/MRP), member 3
• Masse moléculaire calculée: 1527 aa, 169 kDa
• Poids moléculaire observé: 170-180 kDa
• Numéro d’acquisition GenBank: BC137348
• Symbole du gène: MRP3
• Identification du gène (NCBI): 8714
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MRP3 also known as ABCC3, belongs to ATP-binding cassette (ABC) transporter protein superfamily. MRP3 hydrolyzes ATP to enable active transport of various substrates including many drugs, toxicants, and endogenous compounds across cell membranes (PMID: 11581266, 15083066). In normal liver, MRP3 was found present mainly in the bile ducts. In livers of patients with various forms of cholestasis, MRP3 levels were frequently increased in the proliferative cholangiocytes, suggesting its role in cholehepatic and enterohepatic bile circulation and in protecting liver from toxic bile salts( PMID: 11850532, 11283840). MRP3 is expressed in the liver, colon, pancreas, and, at a lower level, in the kidney (PMID:10094960). For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

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