Anticorps Polyclonal de lapin anti-ABCC5

ABCC5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 29792-1-AP

Synonymes

MRP5, MOATC, MOAT C, EC:7.6.2.-, ATP-binding cassette sub-family C member 5

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Galerie de données de validation

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  WB analysis using 29792-1-AP

  Boiled and unboiled MCF-7 lysates were subjected to SDS PAGE followed by western blot with 29792-1-AP (ABCC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29792-1-AP

  Unboiled HepG2 cells and unboiled MCF-7 cells lysates were subjected to SDS PAGE followed by western blot with 29792-1-AP (ABCC5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 29792-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29792-1-AP (ABCC5 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HepG2 non bouillies
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29792-1-AP cible ABCC5 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ABCC5 Protéine recombinante Ag31680
• Nom complet: ATP-binding cassette, sub-family C (CFTR/MRP), member 5
• Masse moléculaire calculée: 161 kDa
• Poids moléculaire observé: 161-200 kDa
• Numéro d’acquisition GenBank: NM_005688
• Symbole du gène: ABCC5
• Identification du gène (NCBI): 10057
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ABCC5, also named as MOAT-C, pABC11, SMRP, and MRP5, belongs to the ABC transporter superfamily, ABCC family, and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump that can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercaptopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site are unclear. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).

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