Anticorps Recombinant de lapin anti-MRPL12

MRPL12 Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242622D12

N° de cat : 85070-5-PBS

Synonymes

242622D12, 5c5-2, Large ribosomal subunit protein bL12m, MRP-L12, MRPL7

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Galerie de données de validation

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  WB analysis using 85070-5-RR (same clone as 85070-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85070-5-RR (MRPL12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 85070-5-RR (same clone as 85070-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85070-5-RR (MRPL12 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 85070-5-RR (same clone as 85070-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 85070-5-RR (MRPL12 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 85070-5-RR (same clone as 85070-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MRPL12 antibody (85070-5-RR, Clone: 242622D12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85070-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85070-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85070-5-RR against Human MRPL12 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

85070-5-PBS cible MRPL12 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MRPL12 Protéine recombinante Ag6504
• Nom complet: mitochondrial ribosomal protein L12
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC002344
• Symbole du gène: MRPL12
• Identification du gène (NCBI): 6182
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian MRPL12 was the first mitochondrial ribosomal protein to be characterized, and is encoded by a nuclear gene. The protein forms a homodimer, localizes predominantly to mitochondria and binds to the large mitochondrial ribosomal subunit [PMID:862670]. It is required for CycD/Cdk4-induced cell growth. In addition, forming a ribosomal stalk with MRPL7, MRPL12 is required for the recruitment of translation elongation factors Tu and G to the large ribosomal subunit [PMID:12370014]. It also a part of a novel regulatory mechanism that coordinates mitochondrial transcription with translation and/or ribosome biogenesis during human mitochondrial gene expression.

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