Anticorps Monoclonal anti-MRPL45

• Phare
• Validé par KD/KO

MRPL45 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B4B4

N° de cat : 66531-1-PBS

Synonymes

L45mt, MRPL45, MRP-L45

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Galerie de données de validation

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  WB analysis using 66531-1-Ig (same clone as 66531-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66531-1-Ig (same clone as 66531-1-PBS)

  WB result of MRPL45 antibody (66531-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL45 transfected HeLa cells. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66531-1-Ig (same clone as 66531-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66531-1-Ig (same clone as 66531-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66531-1-Ig (MRPL45 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66531-1-Ig (same clone as 66531-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66531-1-Ig (MRPL45 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66531-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66531-1-PBS cible MRPL45 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MRPL45 Protéine recombinante Ag8207
• Nom complet: mitochondrial ribosomal protein L45
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC006235
• Symbole du gène: MRPL45
• Identification du gène (NCBI): 84311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL45 is a 39S subunit protein.

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