Anticorps Polyclonal de lapin anti-MRPL49

MRPL49 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 15542-1-AP

Synonymes

C11orf4, Large ribosomal subunit protein mL49, MRP-L49, NOF, NOF1

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Galerie de données de validation

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  WB analysis of BxPC-3 using 15542-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15542-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15542-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15542-1-AP

  IP result of anti-MRPL49 (IP:15542-1-AP, 4ug; Detection:15542-1-AP 1:500) with HeLa cells lysate 3360ug.

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  IHC staining of human breast cancer using 15542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15542-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules A549, cellules HeLa
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below

Informations sur le produit

15542-1-AP cible MRPL49 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPL49 Protéine recombinante Ag7898
• Nom complet: mitochondrial ribosomal protein L49
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC004378
• Symbole du gène: MRPL49
• Identification du gène (NCBI): 740
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.

Publications

Species	Application	Title
human	WB	Mol Cell; Largen: a molecular regulator of mammalian cell size control.; Authors - Kazuo Yamamoto
human	WB	Nucleic Acids Res; C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation.; Authors - Wanschers Bas F J BF
human	WB	Nucleic Acids Res; Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication.; Authors - Nina Rajala
human	WB	Sci Rep; Human Mitochondrial DNA-Protein Complexes Attach to a Cholesterol-Rich Membrane Structure.; Authors - Joachim M Gerhold
mouse	WB	Cell Rep; MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition.; Authors - Jakob D Busch
human	WB	Nucleic Acids Res; Mitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB.; Authors - Fenna Hensen

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