Anticorps Polyclonal de lapin anti-MRPL49

MRPL49 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15542-1-PBS

Synonymes

C11orf4, Large ribosomal subunit protein mL49, MRP-L49, NOF, NOF1

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Galerie de données de validation

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  WB analysis of BxPC-3 using 15542-1-AP (same clone as 15542-1-PBS)

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 15542-1-AP (same clone as 15542-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15542-1-AP (MRPL49 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 15542-1-AP (same clone as 15542-1-PBS)

  IP result of anti-MRPL49 (IP:15542-1-AP, 4ug; Detection:15542-1-AP 1:500) with HeLa cells lysate 3360ug. This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15542-1-AP (same clone as 15542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 15542-1-AP (same clone as 15542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15542-1-AP (MRPL49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15542-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15542-1-PBS cible MRPL49 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPL49 Protéine recombinante Ag7898
• Nom complet: mitochondrial ribosomal protein L49
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 19 kDa
• Numéro d’acquisition GenBank: BC004378
• Symbole du gène: MRPL49
• Identification du gène (NCBI): 740
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MRPL49, also named as C11orf4, NOF1, or SW-cl.67, is a 166 amino acid protein, which belongs to the ribosomal protein L49em family. MRPL49 localizes in the Mitochondrion and is widely expressed in various tissues. MRPL49 is a regulator of C3 convertase of classical pathway.

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