Anticorps Recombinant de lapin anti-MRPL51

• Phare
• Validé par KD/KO

MRPL51 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240381C3

N° de cat : 83565-1-PBS

Synonymes

240381C3, bMRP 64, bMRP64, bMRP-64, CDA09

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Galerie de données de validation

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  WB analysis using 83565-1-RR (same clone as 83565-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83565-1-RR (MRPL51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83565-1-RR (same clone as 83565-1-PBS)

  WB result of MRPL51 antibody (83565-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MRPL51 transfected HEK-293 cells. This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 83565-1-RR (same clone as 83565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 83565-1-RR (same clone as 83565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovarian cancer using 83565-1-RR (same clone as 83565-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83565-1-RR (MRPL51 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 83565-1-RR (same clone as 83565-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using MRPL51 antibody (83565-1-RR, Clone: 240381C3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83565-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83565-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83565-1-RR against Human MRPL51 were performed. The affinity constant is 0.57 nM.

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Informations sur le produit

83565-1-PBS cible MRPL51 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MRPL51 Protéine recombinante Ag7193
• Nom complet: mitochondrial ribosomal protein L51
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC000191
• Symbole du gène: MRPL51
• Identification du gène (NCBI): 51258
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mitochondrial ribosome protein L51 (MRPL51) is a 39S subunit protein of the mitochondrial ribosome. MRPL51 functions in the translation of genetic messages (PMID: 21730110). MRPL51 expression was positively correlated with cell cycle, DNA damage, DNA repair, epithelial-mesenchymal transition, invasion, and proliferation of LUAD cells at the single cell level (PMID: 37323822). The calculated molecular weight of MRPL51 is 15 kDa.

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