Anticorps Monoclonal anti-MRPS27

MRPS27 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B1B3

N° de cat : 66724-1-PBS

Synonymes

FLJ21764, FLJ23348, KIAA0264, MRP S27, MRPS27, S27mt

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Galerie de données de validation

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  WB analysis of LNCaP using 66724-1-Ig (same clone as 66724-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66724-1-Ig (same clone as 66724-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 66724-1-Ig (same clone as 66724-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66724-1-Ig (same clone as 66724-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66724-1-Ig (MRPS27 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66724-1-Ig (same clone as 66724-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66724-1-Ig (MRPS27 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66724-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66724-1-PBS cible MRPS27 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MRPS27 Protéine recombinante Ag11047
• Nom complet: mitochondrial ribosomal protein S27
• Masse moléculaire calculée: 168 aa, 19 kDa, 48 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC030521
• Symbole du gène: MRPS27
• Identification du gène (NCBI): 23107
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Genes encoding PPR (pentatricopeptide repeat)-containing proteins constitute one of the largest gene families in plants. The majority of these proteins are predicted to target organelles and to bind to RNA. Strikingly, there is a dearth of these proteins in mammals, although genomic searches reveal six candidates, all of which are also predicted to target the mitochondrion. One of these proteins, MRPS27is participated in transcription and translation respectively. [PMID:18939947] MRPS27is a component of the small subunit of the mitochondrial ribosome that is encoded by the nuclear genome [PMID: 11279123].

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