Anticorps Polyclonal de lapin anti-MRPS35

MRPS35 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16457-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 16457-1-AP (same clone as 16457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 16457-1-AP (same clone as 16457-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 16457-1-AP (MRPS35 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

  IP result of anti-MRPS35 (IP:16457-1-AP, 4ug; Detection:16457-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16457-1-AP (same clone as 16457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 16457-1-AP (same clone as 16457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16457-1-AP (MRPS35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16457-1-AP (same clone as 16457-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MRPS35 antibody (16457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16457-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16457-1-PBS cible MRPS35 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MRPS35 Protéine recombinante Ag9430
• Nom complet: mitochondrial ribosomal protein S35
• Masse moléculaire calculée: 323 aa, 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC015862
• Symbole du gène: MRPS35
• Identification du gène (NCBI): 60488
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRPS35 (mitochondrial ribosomal protein S35), also known as MDS023, MRPS28 or HDCMD11P, is a 323 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 28S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis. Existing as two alternatively spliced isoforms, MRPS35 is encoded by a gene located on human chromosome 10, which houses over 1,200 genes and comprises nearly 4.5% of the human genome. This antibody is a rabbit polyclonal antibody raised against the full length MRPS35 protein. It specifically reacts with the 37kd MRPS35protein.

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