Anticorps Monoclonal anti-CD20

CD20 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(32)

CloneNo.

4A7G3

N° de cat : 60271-1-Ig

Synonymes

Bp35, B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, B1, B lymphocyte antigen CD20

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Galerie de données de validation

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  WB analysis using 60271-1-Ig

  Raji cells (CD20+), Daudi cells (CD20+), Ramos cells (CD20+), Jurkat cells (CD20-), and HeLa cells (CD20-) were subjected to SDS PAGE followed by western blot with 60271-1-Ig (CD20 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:12800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 60271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 60271-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60271-1-Ig (CD20 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green).

• 

  IF Staining of human tonsillitis using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green).

• 

  IF Staining of human tonsillitis using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CD3 antibody (17617-1-AP, green).

• 

  IF Staining of human tonsillitis using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60271-1-Ig (CD20 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60271-1-Ig (CD20 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig.

• 

  IF Staining of human tonsillitis using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig.

• 

  IF Staining of Raji using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  IF Staining of Raji using 60271-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD20 antibody (60271-1-Ig, Clone: 4A7G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Daudi, cellules Ramos
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu d'appendicite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en IF/ICC: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:15000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 23 publications below
• IF: See 12 publications below

Informations sur le produit

60271-1-Ig cible CD20 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CD20 Protéine recombinante Ag17309
• Nom complet: membrane-spanning 4-domains, subfamily A, member 1
• Masse moléculaire calculée: 297 aa, 33 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC002807
• Symbole du gène: CD20
• Identification du gène (NCBI): 931
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Publications

Species	Application	Title
human	IHC	Clin Transl Med; Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy; Authors - Qing Wang
human	IHC	Chin Med J (Engl); Plasma exchange and intravenous immunoglobulin prolonged the survival of a porcine kidney xenograft in a sensitized, deceased human recipient; Authors - Shuaijun Ma
human	IHC,IF	Int Immunopharmacol; Peritumoral TIGIT+CD20+ B cell infiltration indicates poor prognosis but favorable adjuvant chemotherapeutic response in gastric cancer.; Authors - Huifang Liu
human	IHC	Front Cardiovasc Med; Integrating Bulk Transcriptome and Single-Cell RNA Sequencing Data Reveals the Landscape of the Immune Microenvironment in Thoracic Aortic Aneurysms.; Authors - Qunhui Wang
human	IF	J Pharm Sci; Ultrasound/Magnetic Resonance Bimodal Imaging-Guided CD20-Targeted Multifunctional Nanoplatform for Photothermal/Chemo Synergistic Therapy of B-Cell Lymphoma; Authors - Zhengji Wang
human	IHC	J Endocrinol Invest; The distinctive characteristics of the micro-vasculature and immune cell infiltration in cystic pancreatic neuroendocrine tumors.; Authors - H Gao

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