Anticorps Monoclonal anti-MSH2

• Phare
• Validé par KD/KO

MSH2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

2H5F5

N° de cat : 60161-1-PBS

Synonymes

2H5F5, MSH 2

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Galerie de données de validation

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  WB analysis of HeLa using 60161-1-Ig (same clone as 60161-1-PBS)

  WB results of MSH2 (60161-1-Ig, 1:1000) antibody with si-Control and si-MSH2 transfected HeLa cells.. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  WB analysis using 60161-1-Ig (same clone as 60161-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60161-1-Ig (MSH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50235-1

  Sandwich ELISA standard curve of MP50235-1, Human MSH2 Monoclonal Matched Antibody Pair - PBS only. 60161-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7835. 60161-2-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL

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  Sample test of MP50235-1

  The mean MSH2 concentration was determined to be 10.60 ng/mL in A375 cell extract based on a 2.0 mg/mL extract load and 17.80 ng/mL in A549 cell extract based on a 2.6 mg/mL extract load.

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  IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  IHC staining of Ramos using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Ramos cells slide using 60161-1-Ig (MSH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60161-1-Ig (MSH2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSH2 antibody (60161-1-Ig, Clone: 2H5F5 ) at dilution of 1:2277 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 60161-1-Ig (same clone as 60161-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MSH2 antibody (60161-1-Ig, Clone: 2H5F5 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). This data was developed using the same antibody clone with 60161-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50235-1

  Cytometric bead array standard curve of MP50235-1, MSH2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60161-1-PBS. Detection antibody: 60161-2-PBS. Standard:Ag7835. Range: 0.781-100 ng/mL.

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Informations sur le produit

60161-1-PBS cible MSH2 dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MSH2 Protéine recombinante Ag7835
• Nom complet: mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli)
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC021566
• Symbole du gène: MSH2
• Identification du gène (NCBI): 4436
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MSH2, named for its homologous to the E.coli MutS gene, takes part in DNA mismatch repair(MMR) to maintain the stability of DNA. MSH2 contains a helix-turn-helix domain, which responds for binding to DNA. Also, MSH2 has a direct role in mutation avoidance and micro-satellite stability in cell.

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