Anticorps Recombinant de lapin anti-MSH3

• Phare
• Validé par KD/KO

MSH3 Recombinant Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

1J23

N° de cat : 82845-3-RR

Synonymes

1J23, Divergent upstream protein, DUC1, DUG, hMSH3

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 82845-3-RR

  WB result of MSH3 antibody (82845-3-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MSH3 transfected HEK-293 cells.

• 

  WB analysis using 82845-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82845-3-RR (MSH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 82845-3-RR

  IP result of anti-MSH3 (IP:82845-3-RR, 4ug; Detection:82845-3-RR 1:3000) with HeLa cells lysate 1320 ug.

• 

  IF Staining of U-251 using 82845-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of U2OS using 82845-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MSH3 antibody (82845-3-RR, Clone: 1J23 ) at dilution of 1:1500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

• 

  Affinity and Kinetic Characterization of 82845-3-RR

  Biolayer interferometry (BLl) kinetic assays of 82845-3-RR against Human MSH3 were performed. The affinity constant is below 1 pM.

---

Applications testées

• Résultats positifs en WB: cellules HSC-T6, cellules HEK-293, cellules HEK-293, cellules HeLa, cellules MOLT-4
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF/ICC: cellules U-251, cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82845-3-RR cible MSH3 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MSH3 Protéine recombinante Ag18060
• Nom complet: mutS homolog 3 (E. coli)
• Masse moléculaire calculée: 1137 aa, 127 kDa
• Poids moléculaire observé: 127 kDa
• Numéro d’acquisition GenBank: BC130434
• Symbole du gène: MSH3
• Identification du gène (NCBI): 4437
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MSH3 is a DNA mismatch repair (MMR) gene that heterodimerizes with MSH2 to form MutS beta which binds to DNA mismatches thereby initiating DNA repair. It undergoes frequent somatic mutation in colorectal cancers (CRCs) with MMR deficiency. MSH3 mismatch repair protein regulates sensitivity to cytotoxic drugs and a histone deacetylase inhibitor in human colon carcinoma cells (PMID: 23724141, PMID:32671096).

{{ptg:RelatedPrimaryAntibodies}}