Anticorps Recombinant de lapin anti-MSI1

MSI1 Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251695G8

N° de cat : 86723-1-PBS

Synonymes

Musashi 1, musashi homolog 1 (Drosophila), Musashi-1, RNA-binding protein Musashi homolog 1

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Galerie de données de validation

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  WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86723-1-RR (MSI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

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  WB analysis using 86723-1-RR (same clone as 86723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86723-1-RR (MSI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86723-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP02619-1

  The mean MSI1 concentration was determined to be 12.9 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 9.0 ng/mL in Caco-2 cell extract based on a 1.3 mg/mL extract load, 20.7 ng/mL in HEK-293T cell extract based on a 2.6 mg/mL extract load.

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  Cytometric bead array standard curve of MP02619-1

  Cytometric bead array standard curve of MP02619-1, MSI1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86723-1-PBS. Detection antibody: 86723-2-PBS. Standard: Ag26042. Range: 0.312-40 ng/mL.

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  Affinity and Kinetic Characterization of 86723-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86723-1-RR against Human MSI1 were performed. The affinity constant is 93.8 pM.

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Informations sur le produit

86723-1-PBS cible MSI1 dans les applications de WB, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: MSI1 Protéine recombinante Ag26042
• Nom complet: musashi homolog 1 (Drosophila)
• Masse moléculaire calculée: 362 aa, 39 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC146463
• Symbole du gène: MSI1
• Identification du gène (NCBI): 4440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Musashi1 (Msi1) is an RNA-binding protein expressed in neural progenitor cells and neural stem cells. The gene encoding human Msi1 encodes a 362 amino acid protein. In murine embryonic neural progenitor cells, Msi1 localizes to the cytoplasm and is downregulated during differentiation. Msi1 binds to NUMB, which encodes a membrane-associated antagonist of Notch signaling. Msi1 appears to function in the proliferation and maintenance of stem cell populations of the central nervous system. In addition to its usefulness as a marker for neural progenitor cells in normal human brains, Msi1 is also a marker for human gliomas. In rats, Msi1 is expressed in Sertoli cells of the testis and granulosa cells of the ovary.

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