Anticorps Recombinant de lapin anti-Mesothelin

• Phare
• Validé par KD/KO

Mesothelin Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240795G5

N° de cat : 83744-2-PBS

Synonymes

MSLN, 240795G5, CAK1, CAK1 antigen, Megakaryocyte-potentiating factor

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Galerie de données de validation

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  WB analysis using 83744-2-RR (same clone as 83744-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83744-2-RR (MSLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83744-2-RR (same clone as 83744-2-PBS)

  WB result of Mesothelin antibody (83744-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Mesothelin transfected HeLa cells. This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00697-2

  Sandwich ELISA standard curve of MP00697-2, Human MSLN/Mesothelin Recombinant Matched Antibody Pair - PBS only. 83744-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31658. 83744-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  Sample test of MP00697-2

  Urine of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 923.5 pg/mL with a range of 138.7 - 1,373.8 pg/mL.

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  Sample test of MP00697-2

  Saliva of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 5,273.4 pg/mL with a range of 209.0 - 16,634.9 pg/mL.

• 

  Sample test of MP00697-2

  Serum of four humans was measured. The human MSLN/Mesothelin concentration of detected samples was determined to be 35,341.8 pg/mL with a range of 16,291.9 - 52,246.0 pg/mL.

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  IF Staining of HeLa using 83744-2-RR (same clone as 83744-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 83744-2-RR (same clone as 83744-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83744-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00697-2

  Cytometric bead array standard curve of MP00697-2, MSLN/Mesothelin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83744-5-PBS. Detection antibody: 83744-2-PBS. Standard: Eg31658. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 83744-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83744-2-RR against Human MSLN were performed. The affinity constant is 1.03 nM.

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Informations sur le produit

83744-2-PBS cible Mesothelin dans les applications de WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Mesothelin Protéine recombinante Eg31658
• Nom complet: mesothelin
• Masse moléculaire calculée: 69KD
• Poids moléculaire observé: 69 kDa, 40 kDa
• Numéro d’acquisition GenBank: NM_005823
• Symbole du gène: MSLN
• Identification du gène (NCBI): 10232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

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