Anticorps Recombinant de lapin anti-Mesothelin

• Phare
• Validé par KD/KO

Mesothelin Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

240795G5

N° de cat : 83744-2-RR

Synonymes

MSLN, 240795G5, CAK1, CAK1 antigen, Megakaryocyte-potentiating factor

---

Galerie de données de validation

• 

  WB analysis using 83744-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83744-2-RR (MSLN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 83744-2-RR

  WB result of Mesothelin antibody (83744-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Mesothelin transfected HeLa cells.

• 

  IF Staining of HeLa using 83744-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of THP-1 using 83744-2-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using MSLN antibody (83744-2-RR, Clone: 240795G5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 83744-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83744-2-RR against Human MSLN were performed. The affinity constant is 1.03 nM.

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules BxPC-3, cellules MKN-45
• Résultats positifs en IF/ICC: cellules HeLa, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83744-2-RR cible Mesothelin dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Mesothelin Protéine recombinante Eg31658
• Nom complet: mesothelin
• Masse moléculaire calculée: 69KD
• Poids moléculaire observé: 69 kDa, 40 kDa
• Numéro d’acquisition GenBank: NM_005823
• Symbole du gène: MSLN
• Identification du gène (NCBI): 10232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).

{{ptg:RelatedPrimaryAntibodies}}