Anticorps Polyclonal de lapin anti-Moesin

• Phare
• Validé par KD/KO

Moesin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16495-1-AP

Synonymes

moesin, MSN

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Galerie de données de validation

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  WB analysis using 16495-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 16495-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16495-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16495-1-AP (Moesin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16495-1-AP (Moesin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16495-1-AP (Moesin antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 16495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 16495-1-AP (Moesin antibody) at dilution of 1:200 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IF Staining of HepG2 using 16495-1-AP

  Immunofluorescent analysis of HepG2 cells, using MSN antibody 16495-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 16495-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Moesin antibody (16495-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 16495-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Moesin antibody (16495-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 16495-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Moesin (16495-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules C6, cellules HeLa, cellules Jurkat, cellules NIH/3T3, cellules Raji, cellules SGC-7901
• Résultats positifs en IHC: tissu rénal de souris, tissu cutané humain, tissu ovarien humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16495-1-AP cible Moesin dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Moesin Protéine recombinante Ag9623
• Nom complet: moesin
• Masse moléculaire calculée: 577 aa, 68 kDa
• Poids moléculaire observé: 68-70 kDa
• Numéro d’acquisition GenBank: BC017293
• Symbole du gène: MSN
• Identification du gène (NCBI): 4478
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Moesin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to growth factor, chemokine, and antigen stimulation. This antibody may cross-react with ezrin or radixin with molecular weights around 68-70 kDa.

Publications

Species	Application	Title
human	IHC	Acta Neuropathol Commun; The amyloid plaque proteome in early onset Alzheimer's disease and Down syndrome.; Authors - Eleanor Drummond
human	WB	Int J Mol Sci; Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.; Authors - Krishnendu Khan ; KD Validated
human	WB	Front Cell Dev Biol; Ectopic Endometrial Cell-Derived Exosomal Moesin Induces Eutopic Endometrial Cell Migration, Enhances Angiogenesis and Cytosolic Inflammation in Lesions Contributes to Endometriosis Progression.; Authors - Maidinaimu Abudula
mouse	IF	Am J Pathol; Elevated Expression of Moesin in Muscular Dystrophies.; Authors - Mark Pines