Anticorps Polyclonal de lapin anti-MSRA

MSRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(16)

Avis (1)

N° de cat : 14547-1-AP

Synonymes

Protein-methionine-S-oxide reductase, PMSR, Peptide-methionine, Peptide Met(O) reductase, Mitochondrial peptide methionine sulfoxide reductase

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Galerie de données de validation

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  WB analysis of human kidney using 14547-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 14547-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 14547-1-AP

  IP result of anti-MSRA (IP:14547-1-AP, 4ug; Detection:14547-1-AP 1:600) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human colon using 14547-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 14547-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 14547-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14547-1-AP (MSRA antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, tissu rénal de rat, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

14547-1-AP cible MSRA dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MSRA Protéine recombinante Ag6053
• Nom complet: methionine sulfoxide reductase A
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 24-26 kDa
• Numéro d’acquisition GenBank: BC054033
• Symbole du gène: MSRA
• Identification du gène (NCBI): 4482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methionine sulfoxide reductase A(MSRA), is a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine.MSRA has some isoforms with MW of 19~26 kDa. MSRA functions in the repair of oxidatively damaged proteins to restore biological activity. Transfection of MSRA reduced colony formation and the invasiveness of HCC cell lines.

Publications

Species	Application	Title
	WB	Cell Rep; Inhibiting G6PD by quercetin promotes degradation of EGFR T790M mutation; Authors - Zehe Ge
rat	WB	Oxid Med Cell Longev; Fecal Microbiota Transplantation Is a Promising Method to Restore Gut Microbiota Dysbiosis and Relieve Neurological Deficits after Traumatic Brain Injury.; Authors - Donglin Du
mouse	WB	Free Radic Biol Med; Loss of methionine sulfoxide reductases increases resistance to oxidative stress.; Authors - Lo Lai
human	WB	Proteomics; Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.; Authors - Hao-Yu Li
human	WB	Toxicol Appl Pharmacol; Methionine redox regulation of actin-interacting proteins primarily governs antioxidative signaling and response to the salvianolic acid B treatment in EA.hy926 cells; Authors - Ai-Cheng Wang
rat	WB	J Med Food; Rice Protein Exerts Endogenous Antioxidant Capacity via Methionine Sulfoxide Reductase and the Nrf2 Antioxidant System Independent of Age.; Authors - Hui Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (12-01-2020)

Clear band with clean background. The signal is a little weak.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse liver

MSRA Antibody Western Blot validation (1:500 dilution) in Mouse liver (Cat no:14547-1-AP)

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