Anticorps Polyclonal de lapin anti-MSRA

MSRA Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14547-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis of human kidney using 14547-1-AP (same clone as 14547-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 14547-1-AP (same clone as 14547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14547-1-AP (MSRA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse kidney using 14547-1-AP (same clone as 14547-1-PBS)

  IP result of anti-MSRA (IP:14547-1-AP, 4ug; Detection:14547-1-AP 1:600) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 14547-1-AP (same clone as 14547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14547-1-AP (MSRA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 14547-1-AP (same clone as 14547-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 14547-1-AP (MSRA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14547-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14547-1-PBS cible MSRA dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MSRA Protéine recombinante Ag6053
• Nom complet: methionine sulfoxide reductase A
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 24-26 kDa
• Numéro d’acquisition GenBank: BC054033
• Symbole du gène: MSRA
• Identification du gène (NCBI): 4482
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Methionine sulfoxide reductase A(MSRA), is a ubiquitous and highly conserved protein that carries out the enzymatic reduction of methionine sulfoxide to methionine.MSRA has some isoforms with MW of 19~26 kDa. MSRA functions in the repair of oxidatively damaged proteins to restore biological activity. Transfection of MSRA reduced colony formation and the invasiveness of HCC cell lines.

{{ptg:RelatedPrimaryAntibodies}}