Anticorps Polyclonal de lapin anti-MT3

MT3 Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12179-1-AP

Synonymes

GIF, GIFB, GRIF, Growth inhibitory factor, metallothionein 3, Metallothionein III, MT 3, MT III, MT3

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Galerie de données de validation

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  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12179-1-AP (MT3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12179-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12179-1-AP (MT3 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

12179-1-AP cible MT3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MT3 Protéine recombinante Ag2823
• Nom complet: metallothionein 3
• Masse moléculaire calculée: 68 aa, 7 kDa
• Numéro d’acquisition GenBank: BC013081
• Symbole du gène: MT3
• Identification du gène (NCBI): 4504
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MT3(Metallothionein-3) is also names as GIF(Growth inhibitory factor),GIFB inhibits N-glycosylation of IgE-binding factors and that unglycosylated IgE-binding factors selectively suppressed IgE synthesis.The major cell source of GIF is antigen-specific suppressor T(Ts)cell(PMID:10967025).The molecular size of GIF from T cells is 15 kDa, while GIF from macrophages consisted of 40-and 15-kDa molecules(PMID:2417235).

Publications

Species	Application	Title
pig		Int J Mol Sci; Integrating Transcriptomics and Proteomics to Characterize the Intestinal Responses to Cadmium Exposure Using a Piglet Model; Authors - Yikun Li
mouse	WB	Biochem Biophys Res Commun; Upregulation of p52-ZER6 (ZNF398) increases reactive oxygen species by suppressing metallothionein-3 in neuronal cells; Authors - Eunsang Kwag

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