Anticorps Polyclonal de lapin anti-MTA3

• Phare
• Validé par KD/KO

MTA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14682-1-PBS

Synonymes

Metastasis-associated protein MTA3, MTA 3, mta1

---

Galerie de données de validation

• 

  WB analysis using 14682-1-AP (same clone as 14682-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 14682-1-AP (same clone as 14682-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14682-1-AP (MTA3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Raji using 14682-1-AP (same clone as 14682-1-PBS)

  IP result of anti-MTA3 (IP:14682-1-AP, 4ug; Detection:14682-1-AP 1:3000) with Raji cells lysate 2840 ug. This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14682-1-AP (same clone as 14682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 14682-1-AP (same clone as 14682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14682-1-AP (MTA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 14682-1-AP (same clone as 14682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 14682-1-AP (same clone as 14682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MTA3 antibody (14682-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 14682-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

14682-1-PBS cible MTA3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTA3 Protéine recombinante Ag6400
• Nom complet: metastasis associated 1 family, member 3
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 62 kDa, 68 kDa
• Numéro d’acquisition GenBank: BC053631
• Symbole du gène: MTA3
• Identification du gène (NCBI): 57504
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The metastasis-associated (MTA) family contains three members, MTA1, MTA2 and MTA3 (PMID:12167865).Metastasis-associated protein 3 (MTA3) is a cell-type specific component of the Mi-2-NURD transcriptional co-repressor complex. It has a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels.

{{ptg:RelatedPrimaryAntibodies}}