Anticorps Monoclonal anti-MTAP

MTAP Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2B1G6

N° de cat : 66706-1-Ig

Synonymes

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 66706-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 66706-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66706-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66706-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66706-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 66706-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66706-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTAP antibody (66706-1-Ig, Clone: 2B1G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HCT 116, cellules HSC-T6, cellules HT-29, cellules Raji
• Résultats positifs en IHC: tissu hépatique de souris, tissu de cancer du côlon humain, tissu de cancer du foie humain, tissu hépatique de rat, tissu rénal de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66706-1-Ig cible MTAP dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTAP Protéine recombinante Ag17954
• Nom complet: methylthioadenosine phosphorylase
• Masse moléculaire calculée: 283 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC018625
• Symbole du gène: MTAP
• Identification du gène (NCBI): 4507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.