Anticorps Monoclonal anti-MTAP

MTAP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B1G6

N° de cat : 66706-1-PBS

Synonymes

2B1G6, 5'-methylthioadenosine phosphorylase, c86fus, EC:2.4.2.28, MSAP

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 66706-1-Ig (same clone as 66706-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 66706-1-Ig (same clone as 66706-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66706-1-Ig (same clone as 66706-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 66706-1-Ig (same clone as 66706-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66706-1-Ig (same clone as 66706-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66706-1-Ig (MTAP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66706-1-Ig (MTAP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66706-1-Ig (same clone as 66706-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MTAP antibody (66706-1-Ig, Clone: 2B1G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66706-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66706-1-PBS cible MTAP dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: MTAP Protéine recombinante Ag17954
• Nom complet: methylthioadenosine phosphorylase
• Masse moléculaire calculée: 283 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC018625
• Symbole du gène: MTAP
• Identification du gène (NCBI): 4507
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.

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